Leitch H A, Yip S T, Jamieson C H, Levy J G
Department of Microbiology, University of British Columbia, Vancouver, Canada.
Leukemia. 1993 May;7(5):717-24.
CAMAL (common antigen of myelogenous acute leukemia) is an antigenic preparation isolated in this laboratory from the bone marrow or peripheral blood leucocytes of persons with myeloid leukemias and shown using an immunoperoxidase slide test to be diagnostic of these leukemias. Material further purified from CAMAL preparations, which migrates in the range of 30-35 kilodaltons (kDa) by sodium dodecyl sulphate/polyacrylamide gel electrophoresis (SDS-PAGE), which is referred to as P30-35 CAMAL, and was previously shown to be inhibitory to colony formation by progenitor cells from normal healthy human donors in vitro. This inhibitory activity was directed toward neutrophilic granulocyte colonies (CFU-G) in particular. We now report that P30-35 CAMAL is inhibitory to colony formation by murine progenitor cells in vitro. Colonies from P30-35 CAMAL-treated cultures of murine bone marrow cells were reduced in number and in size, an effect similar to that seen in cultures of human cells. As in assays using human cells, murine CFU-G appeared to be preferentially targeted by the inhibitory activity of P30-35 CAMAL. In addition, day 10 spleen colony formation was inhibited by P30-35 CAMAL in an ex vivo assay. Hence, the effects of P30-35 CAMAL on murine progenitor cells appear to parallel the effects observed using human cells. These observations support the possibility that CAMAL might be a regulatory protein in hematopoiesis which is conserved between species.
髓性急性白血病共同抗原（CAMAL）是本实验室从髓性白血病患者的骨髓或外周血白细胞中分离出的一种抗原制剂，通过免疫过氧化物酶玻片试验显示其可用于诊断这些白血病。从CAMAL制剂中进一步纯化得到的物质，经十二烷基硫酸钠/聚丙烯酰胺凝胶电泳（SDS-PAGE）分析，其迁移范围在30 - 35千道尔顿（kDa）之间，被称为P30 - 35 CAMAL，此前已证明它在体外对正常健康人类供体的祖细胞集落形成具有抑制作用。这种抑制活性尤其针对嗜中性粒细胞集落（CFU - G）。我们现在报告，P30 - 35 CAMAL在体外对小鼠祖细胞的集落形成具有抑制作用。经P30 - 35 CAMAL处理的小鼠骨髓细胞培养物形成的集落在数量和大小上均减少，这一效应与在人类细胞培养物中观察到的相似。与使用人类细胞的试验一样，小鼠CFU - G似乎是P30 - 35 CAMAL抑制活性的优先作用靶点。此外，在一项体外试验中，P30 - 35 CAMAL抑制了第10天脾脏集落的形成。因此，P30 - 35 CAMAL对小鼠祖细胞的作用似乎与在人类细胞中观察到的作用相似。这些观察结果支持了CAMAL可能是一种在造血过程中保守的物种间调节蛋白的可能性。
