Muegge K, West M, Durum S K
Biological Carcinogenesis and Development Program, Program Resources, Inc.,/Dyncorp, National Cancer Institute-Frederick Cancer Research and Development Center, MD 21702-1201.
Proc Natl Acad Sci U S A. 1993 May 1;90(9):4151-5. doi: 10.1073/pnas.90.9.4151.
Rearrangement of T-cell antigen receptor and immunoglobulin genes occurs in immature lymphoid cells by an unknown mechanism. To identify components of the rearrangement machinery, we isolated a population of murine thymocytes enriched for rearranging pre-T cells. In the nuclear fraction of these cells, we detected a protein that specifically bound the recombination sequences that flank T-cell receptor and immunoglobulin genes and are required for their rearrangement. This protein recognized both heptamer and nonamer motifs of the recombination sequence, separated by either 12 or 23 bp. The protein complexed with the recombination sequence oligonucleotide had an apparent molecular mass of 30 kDa. The binding characteristics of the protein and its presence in rearranging thymocytes and cell lines suggest that it could serve as the recognition unit of a recombinase complex.
T细胞抗原受体和免疫球蛋白基因的重排通过一种未知机制在未成熟淋巴细胞中发生。为了鉴定重排机制的组成成分，我们分离出了一群富含重排前T细胞的小鼠胸腺细胞。在这些细胞的核部分，我们检测到一种蛋白质，它能特异性结合T细胞受体和免疫球蛋白基因两侧的重组序列，这些序列是它们重排所必需的。这种蛋白质识别重组序列的七聚体和九聚体基序，它们被12或23个碱基对隔开。与重组序列寡核苷酸复合的蛋白质的表观分子量为30 kDa。该蛋白质的结合特性及其在重排胸腺细胞和细胞系中的存在表明，它可能作为重组酶复合物的识别单位。
