Wasniowska K, Eichenberger P, Kugele F, Hadley T J
University of Louisville School of Medicine, KY.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):366-72. doi: 10.1006/bbrc.1993.1424.
Duffy blood group antigenic epitopes are located on a 35-43 kD integral membrane protein of the erythrocyte membrane. This protein functions as a receptor for the human malaria parasite, Plasmodium vivax. The Duffy protein has been difficult to purify because of its tendency to form aggregates. Here we describe purification of a 28 kD tryptic fragment of the Duffy protein and purification of an 18 kD de-glycosylated form of the Duffy tryptic peptide using Thiopropyl Sepharose 6B chromatography and preparative SDS-PAGE. These Duffy-reactive peptides do not form aggregates and may prove amendable to protein sequencing.
达菲血型抗原表位位于红细胞膜上一种35 - 43千道尔顿的整合膜蛋白上。该蛋白作为人类疟原虫间日疟原虫的受体发挥作用。由于达菲蛋白易于形成聚集体，因此很难进行纯化。在此，我们描述了使用硫丙基琼脂糖6B层析和制备性SDS - 聚丙烯酰胺凝胶电泳对达菲蛋白的一个28千道尔顿胰蛋白酶片段以及达菲胰蛋白酶肽的一种18千道尔顿去糖基化形式进行纯化的过程。这些与达菲反应的肽不会形成聚集体，可能适合进行蛋白质测序。
