Barker S, Marchant W, Clark A J, Jimenez E, Marsigliante S, Montiel M, Vinson G P
Department of Biochemistry, Faculty of Basic Medical Sciences, Queen Mary and Westfield College, London, U.K.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):392-8. doi: 10.1006/bbrc.1993.1428.
Rat adrenal AT1A and AT1B receptors from transfected COS-7 cells were labelled with 125I-Angiotensin II, solubilised, and run on isoelectric focusing gels. Receptors from rat tissues were treated similarly. COS-7 cell-expressed AT1A and AT1B receptors each produced a single peak of specific radioactivity at pI 6.8. Rat liver and rat ovary tissue preparations gave peaks at pI 6.8 and 6.5, respectively. In contrast, rat adrenal tissue preparations gave four peaks at pI 7.0, 6.8, 6.5, and 6.3. The additional isoforms found in the rat adrenal tissue preparations may represent post-translationally modified or novel receptors.
用125I-血管紧张素II标记转染的COS-7细胞中的大鼠肾上腺AT1A和AT1B受体，使其溶解，并在等电聚焦凝胶上进行电泳。大鼠组织中的受体也进行类似处理。COS-7细胞表达的AT1A和AT1B受体在pI 6.8处均产生一个特异性放射性单峰。大鼠肝脏和大鼠卵巢组织提取物分别在pI 6.8和6.5处出现峰。相比之下，大鼠肾上腺组织提取物在pI 7.0、6.8、6.5和6.3处出现四个峰。在大鼠肾上腺组织提取物中发现的额外异构体可能代表翻译后修饰的受体或新受体。
