Yokoyama-Kobayashi M, Kato S
Kanagawa Academy of Science and Technology, Japan.
Biochem Biophys Res Commun. 1993 Apr 30;192(2):935-9. doi: 10.1006/bbrc.1993.1505.
Recombinant f1 phage particles containing cDNA of the human urokinase type-plasminogen activator (u-PA) under the control of the simian virus 40 (SV40) early promoter were used for transfection of monkey COS-7 cells using a DEAE dextran method. The fibrinolytic activity of u-PA was detected in the culture medium of the 10(5) cells transfected with the phage particles containing single-stranded (ss) DNA of more than 0.2 ng. This finding will lead us to develop a simple and efficient method for expression cloning using mammalian cells.
含有在猿猴病毒40（SV40）早期启动子控制下的人尿激酶型纤溶酶原激活剂（u-PA）cDNA的重组f1噬菌体颗粒，采用DEAE葡聚糖法用于转染猴COS-7细胞。在用含有超过0.2 ng单链（ss）DNA的噬菌体颗粒转染的10⁵个细胞的培养基中检测到了u-PA的纤溶活性。这一发现将引导我们开发一种使用哺乳动物细胞进行表达克隆的简单高效方法。
