Thomas J L, Pourquie O, Coltey M, Vaigot P, Le Douarin N M
Institut d'Embryologie cellulaire et moléculaire du CNRS, Nogent-sur-Marne, France.
Exp Cell Res. 1993 Jan;204(1):156-66. doi: 10.1006/excr.1993.1020.
We report the production of two monoclonal antibodies reacting, respectively, with a 92-kDa protein (GRL1) and a 40- to 65-kDa membrane glycoprotein (GRL2), both present in chicken thrombocyte and myelocyte granules. We examined the expression of GRL1 and GRL2 during the development of the hematopoietic system: GRL1 is restricted to thrombocytes and myelocytes, whereas GRL2 is present in thrombocytes, myelocytes, myeloid progenitors, and a subpopulation of erythroid progenitors. In the lymphoid lineages, neither GRL1 nor GRL2 is expressed during thymus and bursa ontogeny or on resting peripheral blood lymphocytes. However, CD3+ T lymphoblasts obtained by mitogenic stimulation of GRL2-negative quiescent T lymphocytes are stained on their surface by anti-GRL2 Mab. In vitro stimulation of thrombocytes and granulocytes with their specific secretagogues results in the expression of GRL1 and in the overexpression of GRL2 on the cell surface. These observations are consistent with the following two conclusions: the presence on the cell surface of GRL1 epitope is a marker of thrombocyte and myelocyte activation; GRL2 epitope is present on the granule membrane of leukocytes, including T cells. In that respect, GRL2 appears to share certain features with leukocyte activation antigens recently described in human.
我们报告了两种单克隆抗体的产生，它们分别与一种92-kDa蛋白（GRL1）和一种40至65-kDa的膜糖蛋白（GRL2）发生反应，这两种蛋白都存在于鸡的血小板和髓细胞颗粒中。我们研究了造血系统发育过程中GRL1和GRL2的表达情况：GRL1仅限于血小板和髓细胞，而GRL2存在于血小板、髓细胞、髓系祖细胞和一部分红系祖细胞中。在淋巴谱系中，GRL1和GRL2在胸腺和法氏囊发育过程中或静息外周血淋巴细胞上均不表达。然而，通过有丝分裂刺激GRL2阴性的静止T淋巴细胞获得的CD3+ T淋巴母细胞，其表面可被抗GRL2单克隆抗体染色。用其特异性促分泌剂对血小板和粒细胞进行体外刺激，会导致GRL1的表达以及GRL2在细胞表面的过表达。这些观察结果与以下两个结论一致：GRL1表位在细胞表面的存在是血小板和髓细胞活化的标志物；GRL2表位存在于包括T细胞在内的白细胞颗粒膜上。在这方面，GRL2似乎与最近在人类中描述的白细胞活化抗原有某些共同特征。
