一种编码假定的NAD依赖性亚甲基四氢叶酸脱氢酶-亚甲基四氢叶酸环水解酶的果蝇基因的分离与鉴定。
The isolation and characterization of a Drosophila gene encoding a putative NAD-dependent methylenetetrahydrofolate dehydrogenase-methenyltetrahydrofolate cyclohydrolase.
作者信息
Price B D, Laughon A
机构信息
Laboratory of Genetics, University of Wisconsin, Madison 53706.
出版信息
Biochim Biophys Acta. 1993 Apr 29;1173(1):94-8. doi: 10.1016/0167-4781(93)90251-8.
Mammalian NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase-5,10-methenyltetrahydrofolate cyclohydrolase is a bifunctional mitochondrial enzyme expressed in most established cell lines but only in developing normal tissues. We report the cloning and molecular characterization of a Drosophila gene (DNMDMC) that encodes a protein with 56% identity to the mammalian bifunctional protein. Like the mammalian bifunctional proteins, the Drosophila protein contains a putative mitochondrial targeting sequence and its transcripts are expressed in developing tissues. Unlike its mammalian homologs, DNMDMC is expressed at high levels in adult tissues. DNMDMC maps to polytene chromosome band 85C, is encoded in three exons, and is closely flanked by two additional genes.
哺乳动物依赖烟酰胺腺嘌呤二核苷酸（NAD）的5,10-亚甲基四氢叶酸脱氢酶-5,10-亚甲四氢叶酸环水解酶是一种双功能线粒体酶，在大多数已建立的细胞系中表达，但仅在发育中的正常组织中表达。我们报告了果蝇基因（DNMDMC）的克隆和分子特征，该基因编码一种与哺乳动物双功能蛋白具有56%同源性的蛋白质。与哺乳动物双功能蛋白一样，果蝇蛋白含有一个推定的线粒体靶向序列，其转录本在发育中的组织中表达。与哺乳动物同源物不同，DNMDMC在成年组织中高水平表达。DNMDMC定位于多线染色体带85C，由三个外显子编码，并紧密相邻另外两个基因。
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