[低强度氦氖激光作用下大肠杆菌1257细菌细胞中腺嘌呤核苷酸池的变化]
[Change in adenine nucleotide pool in E. coli 1257 bacterial cells under the action of a low intensity He-Ne laser].
作者信息
Romanova N A, Brovko L Iu, Sepul'veda-Bessera M A, Ugarova N N
出版信息
Biokhimiia. 1993 Mar;58(3):376-84.
PMID:8485225
Abstract
Evidence is presented of co-immobilized bioluminescent reagents which include firefly luciferase, pyruvate kinase and adenylate kinase used for an adenylate assay in bacterial cells. The changes in the ATP, ADP and AMP content induced by irradiation with a low-power He-Ne-laser depend on the laser power and irradiation dose. The data obtained suggest that laser irradiation causes the activation of adenine nucleotide synthesis de novo.
摘要
有证据表明，共固定化的生物发光试剂包括萤火虫荧光素酶、丙酮酸激酶和腺苷酸激酶，用于细菌细胞中的腺苷酸测定。低功率氦氖激光照射引起的ATP、ADP和AMP含量变化取决于激光功率和照射剂量。所得数据表明，激光照射会导致腺嘌呤核苷酸从头合成的激活。
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