Chikhirzhina G I, Fedorova S A, Chespokov I N, Romanovskaia E V
Biokhimiia. 1993 Mar;58(3):392-8.
Using electrophoretic mobility shift assay we have analysed the binding of rat liver nuclear proteins to the fragments from -466 to -292 and from -292 to -178 relative to the transcriptional start site of the rat tryptophan oxygenase gene. Studies of the competition with a synthetic consensus sequence for the NF 1 recognition site have shown that the liver nuclear proteins, responsible for the formation of specific complexes with these fragments belong to the family of the nuclear factor 1 (NF 1). We have also found that the trans-acting factors of the NF 1 family form several complexes with each of the two tested fragments of the tryptophan oxygenase gene.
我们使用电泳迁移率变动分析，分析了大鼠肝脏核蛋白与相对于大鼠色氨酸加氧酶基因转录起始位点从-466至-292以及从-292至-178的片段的结合情况。与NF 1识别位点的合成共有序列进行竞争研究表明，负责与这些片段形成特异性复合物的肝脏核蛋白属于核因子1（NF 1）家族。我们还发现，NF 1家族的反式作用因子与色氨酸加氧酶基因的两个测试片段中的每一个都形成了几种复合物。
