Godard A, Fauchet F, Raher S, Jadoul M, Thuillier B, Dehart J, Soulillou J P, Baudrihaye M, Jacques Y, De Groote D
INSERM U.211 (Unité de Recherche sur les Effecteurs Lymphocytaires T), C.H.U. Nantes, France.
Cytokine. 1993 Jan;5(1):16-23. doi: 10.1016/1043-4666(93)90019-2.
An adoptive transfer immunization/fusion protocol in mice has been successfully used to raise a series of monoclonal antibodies directed against the Human Interleukin for DA-1a (HILDA)/Leukemia Inhibitory Factor (LIF) cytokine. These antibodies which were raised using recombinant HILDA/LIF purified from conditioned medium of transfected Chinese Hamster Ovary (CHO) cells also react with natural HILDA/LIF from the HSB2 T lymphoma cell line and unglycosylated HILDA/LIF produced in E. coli. They define four separate epitopes, one of which is involved in receptor binding and induction of biological activity. A sensitive sandwich immunoradiometric assay which is linear up to 5 ng/ml HILDA/LIF and can detect as low as 25 pg/ml of the cytokine has been developed.
小鼠中的过继转移免疫/融合方案已成功用于产生一系列针对人白细胞介素DA-1a（HILDA）/白血病抑制因子（LIF）细胞因子的单克隆抗体。这些抗体是使用从转染的中国仓鼠卵巢（CHO）细胞的条件培养基中纯化的重组HILDA/LIF产生的，它们也与HSB2 T淋巴瘤细胞系中的天然HILDA/LIF以及大肠杆菌中产生的未糖基化HILDA/LIF发生反应。它们定义了四个独立的表位，其中一个表位参与受体结合和生物活性诱导。已经开发出一种灵敏的夹心免疫放射分析方法，该方法在高达5 ng/ml的HILDA/LIF浓度范围内呈线性，并且能够检测低至25 pg/ml的细胞因子。
