Pan A, Tie F, Duan Z, Ma H, Li L, Ru B
Department of Biology, Peking University, Beijing, China.
Biomed Chromatogr. 1993 Mar-Apr;7(2):94-8. doi: 10.1002/bmc.1130070210.
Two forms of liver metallothioneins (MTs) were purified from hedgehog exposed to zinc, using gel filtration on Sephacryl S-100 and DEAE Sepharose Fast Flow chromatography. The peptide chain weight of both MT-1 and MT-2 was found to be about 10,000, as determined by high performance liquid chromatography. This value was higher than that calculated from amino acid analysis. The amino acid composition of hedgehog liver MT-1 and MT-2 resembles that of liver to MTs from rabbit and other species. Their distinctive features include an extremely high cysteine content, about 33% of all the amino acid residues, and an absence of aromatic amino acids and histidine. In addition, a rapid method for the determination of MTs during animal tissue purification has been established. The samples were directly added in an ammoniacal solution of a Co(II) salt for recording linear sweep polarograms. By comparison with the commonly used metal determination method, our method is direct, rapid, credible and suitable for all the MTs or MT-like samples.
采用Sephacryl S - 100凝胶过滤和DEAE Sepharose Fast Flow色谱法，从暴露于锌的刺猬中纯化出两种形式的肝脏金属硫蛋白（MTs）。通过高效液相色谱法测定，MT - 1和MT - 2的肽链分子量均约为10,000。该值高于根据氨基酸分析计算得出的值。刺猬肝脏MT - 1和MT - 2的氨基酸组成与兔及其他物种肝脏MTs的氨基酸组成相似。它们的显著特征包括半胱氨酸含量极高，约占所有氨基酸残基的33%，且不含芳香族氨基酸和组氨酸。此外，还建立了一种在动物组织纯化过程中测定MTs的快速方法。将样品直接加入钴（II）盐的氨溶液中记录线性扫描极谱图。与常用的金属测定方法相比，我们的方法直接、快速、可靠，适用于所有MTs或MT样样品。
