Kuromi H
Department of Neuropharmacology, School of Medicine, Chiba University, Japan.
Brain Res Dev Brain Res. 1993 Apr 16;72(2):159-66. doi: 10.1016/0165-3806(93)90181-9.
A protein named 'sympathonectin', was purified from chick heart cell-conditioned medium (HCM), on the basis of its ability to direct the neurite outgrowth of cultured sympathetic neurons. The most purified fraction showed a doublet band on SDS-PAGE with an apparent molecular weight of 370 kDa. The biological activity of sympathonectin was over 100 times higher than that of laminin. Immunoblotting with anti-sympathonectin of the 100,000 x g pellet (particulate) fraction of HCM identified a distinct 370 kDa band; this molecule did not react to the anti-laminin serum. The immunohistochemical analysis showed that the antibody against sympathonectin stained heart tissue but not skeletal muscle tissue, whereas anti-laminin serum stained both tissues. These results suggest that sympathonectin may play a role during sympathetic innervation of target organs.
一种名为“交感连接蛋白”的蛋白质，是从鸡心脏细胞条件培养基（HCM）中纯化出来的，其依据是它能够引导培养的交感神经元的轴突生长。最纯的组分在SDS-PAGE上显示为一条双峰带，表观分子量为370 kDa。交感连接蛋白的生物活性比层粘连蛋白高100倍以上。用抗交感连接蛋白抗体对HCM的100,000×g沉淀（颗粒）组分进行免疫印迹，鉴定出一条明显的370 kDa条带；该分子不与抗层粘连蛋白血清发生反应。免疫组织化学分析表明，抗交感连接蛋白抗体可使心脏组织染色，但不能使骨骼肌组织染色，而抗层粘连蛋白血清可使两种组织都染色。这些结果表明，交感连接蛋白可能在靶器官的交感神经支配过程中发挥作用。
