用31P核磁共振研究抗肿瘤活性金属茂二氯二茂钼（Cp2MoCl2）与小牛胸腺DNA的相互作用。

A 31P NMR study of the interaction of the antitumor active metallocene Cp2MoCl2 with calf thymus DNA.

作者信息

Harding M M, Harden G J, Field L D

机构信息

Department of Organic Chemistry, University of Sydney, N.S.W., Australia.

出版信息

FEBS Lett. 1993 May 17;322(3):291-4. doi: 10.1016/0014-5793(93)81588-q.

DOI:10.1016/0014-5793(93)81588-q

PMID:8486161

Abstract

Treatment of sonicated calf thymus DNA with the antitumor active metallocene Cp2MoCl2 afforded a metallocene-DNA complex which was characterized by 31P NMR spectroscopy. In addition to the resonance for the phosphate backbone (delta-1.6), the spectrum contained 2 signals assigned to a phosphate bound Mo-DNA complex(es) (delta 37.2, 36.5) and a broad signal at delta 6.2 ppm. This result suggests that covalent attachment of the metallocene Cp2MoCl2 occurs via phosphate(O) coordination and is accompanied by local distortion of the DNA backbone. This result supports recent ICP studies with Cp2TiCl2 that have DNA detected DNA-metallocene adducts.

摘要

用具有抗肿瘤活性的金属茂二氯二茂钼（Cp2MoCl2）处理超声处理过的小牛胸腺DNA，得到了一种金属茂-DNA复合物，该复合物通过31P核磁共振光谱进行了表征。除了磷酸主链的共振信号（δ -1.6）外，光谱中还包含两个归属于与钼结合的DNA复合物的磷酸信号（δ 37.2、36.5）以及一个位于δ 6.2 ppm处的宽信号。该结果表明，金属茂Cp2MoCl2通过磷酸（O）配位发生共价连接，并伴随着DNA主链的局部扭曲。这一结果支持了最近关于二氯二茂钛（Cp2TiCl2）的电感耦合等离子体质谱（ICP）研究，该研究已检测到DNA-金属茂加合物。