Furones M D, Gilpin M L, Munn C B
Department of Biological Sciences, University of Plymouth, UK.
J Appl Bacteriol. 1993 Apr;74(4):360-6. doi: 10.1111/j.1365-2672.1993.tb05139.x.
Strains of the bacterial fish pathogen Yersinia ruckeri were identified with the API 20E system and distinguished on the basis of whole cell agglutination with antisera, sorbitol fermentation and polymyxin B sensitivity. Strains which were shown to possess the virulence-associated heat-sensitive factor (HSF) were shown to grow preferentially on culture media containing sodium dodecyl sulphate (SDS) and to produce a creamy deposit around the colonies. By contrast, strains lacking this factor (HSF-) grew poorly and without forming a deposit. Enhancement of the differentiation between the two types was shown by the incorporation of Coomassie brilliant blue dye into agar containing 1% SDS, and the uptake of Coomassie blue and Congo red was shown to be temperature-dependent. Most strains tested were shown to belong to serotype I, and were sensitive to polymyxin and did not ferment sorbitol. With the medium developed most serotype I strains but not those of other serotypes were shown to possess HSF. It is suggested that the medium is used in epidemiological studies of Y. ruckeri.
采用API 20E系统对细菌性鱼类病原菌鲁氏耶尔森菌菌株进行鉴定，并根据与抗血清的全细胞凝集反应、山梨醇发酵和对多粘菌素B的敏感性进行区分。已证明具有与毒力相关的热敏感因子（HSF）的菌株在含有十二烷基硫酸钠（SDS）的培养基上优先生长，并在菌落周围产生乳脂状沉淀。相比之下，缺乏该因子的菌株（HSF-）生长不良且不形成沉淀。通过在含有1% SDS的琼脂中加入考马斯亮蓝染料，显示出两种类型之间的差异增强，并且考马斯亮蓝和刚果红的摄取显示出温度依赖性。大多数测试菌株属于血清型I，对多粘菌素敏感且不发酵山梨醇。使用所开发的培养基，大多数血清型I菌株显示具有HSF，而其他血清型的菌株则没有。建议将该培养基用于鲁氏耶尔森菌的流行病学研究。
