Droke E A, Paape M J, Di Carlo A L
Agricultural Research Service, USDA, Beltsville, MD 20705.
J Dairy Sci. 1993 Apr;76(4):1035-9. doi: 10.3168/jds.S0022-0302(93)77431-7.
Higher SCC have been observed in goat milk than in cow milk. This study evaluated the prevalence of high SCC and determined whether increased neutrophils were a factor in high SCC. Bulk tank goat milk samples were collected from commercial dairy goat herds in California, Arkansas, Michigan, and Wisconsin. Total SCC were determined using a Fossomatic Electronic Cell Counter and microscopically using pyronin Y-methyl green stain. Differential counts were determined using Wright's-stained cytospin centrifuge preparations. Average SCC were high (6.12 log10 cells/ml; geometric mean, 1.32 x 10(6) cells/ml). The average of the Fossomatic and direct microscopic SCC indicated that 8.6% of the producers had SCC < .75 x 10(6) cells/ml and that 34.5% were < 1 x 10(6) cells/ml. The current goat milk SCC standard is 1 x 10(6) cells/ml; the cow milk SCC standard will be .75 x 10(6) cells/ml in 1993. Milk samples contained 9.9% macrophages, 2.8% lymphocytes, and 87% neutrophils. These data indicate that high SCC were prevalent and that increased neutrophils contributed to high SCC in the bulk tank goat milk.
已观察到山羊奶中的体细胞数(SCC)高于牛奶。本研究评估了高SCC的患病率,并确定嗜中性粒细胞增多是否是高SCC的一个因素。从加利福尼亚州、阿肯色州、密歇根州和威斯康星州的商业奶山羊群中采集了大罐山羊奶样本。使用Fossomatic电子细胞计数器测定总SCC,并使用派洛宁Y-甲基绿染色进行显微镜检查。使用瑞氏染色的细胞离心涂片制剂进行分类计数。平均SCC较高(6.12 log10个细胞/毫升;几何平均值,1.32×10⁶个细胞/毫升)。Fossomatic和直接显微镜检查SCC的平均值表明,8.6%的生产者的SCC<0.75×10⁶个细胞/毫升,34.5%的生产者的SCC<1×10⁶个细胞/毫升。目前山羊奶的SCC标准是1×10⁶个细胞/毫升;牛奶的SCC标准在1993年将为0.75×10⁶个细胞/毫升。奶样中含有9.9%的巨噬细胞、2.8%的淋巴细胞和87%的嗜中性粒细胞。这些数据表明,高SCC很普遍,且嗜中性粒细胞增多导致了大罐山羊奶中SCC升高。