Morris G E, Ellis J M, Nguyen T M
Research Division, N.E. Wales Institute, Deeside, Clwyd, UK.
J Immunol Methods. 1993 May 5;161(1):23-8. doi: 10.1016/0022-1759(93)90194-c.
A novel approach to the quantitation of the muscular dystrophy protein, dystrophin, in muscle extracts is described. The two-site ELISA uses two monoclonal antibodies against dystrophin epitopes which lie close together in the rod domain of the dystrophin molecule in order to minimize the effects of dystrophin degradation. Dystrophin is assayed in its native form by extracting with non-ionic detergents and avoiding the use of SDS.
本文描述了一种定量肌肉提取物中肌营养不良蛋白（抗肌萎缩蛋白）的新方法。双位点酶联免疫吸附测定（ELISA）使用两种针对抗肌萎缩蛋白表位的单克隆抗体，这些表位在抗肌萎缩蛋白分子的杆状结构域中彼此靠近，以尽量减少抗肌萎缩蛋白降解的影响。通过用非离子去污剂提取并避免使用十二烷基硫酸钠（SDS），以天然形式测定抗肌萎缩蛋白。
