Prosperi E, Stivala L A, Bianchi L
Centro di Studio per l'Istochimica del CNR, Pavia, Italy.
J Photochem Photobiol B. 1993 Apr;18(1):91-3. doi: 10.1016/1011-1344(93)80045-b.
The sensitivity of human fibroblast DNA towards the activity of nuclease S1 was investigated in situ after irradiation of the cells with UVC light. DNA digestion was assessed using flow cytometry by staining the nuclease-resistant DNA fraction with the specific fluorochrome propidium iodide (PI). The results showed a non-linear dependence of DNA digestion on the UVC irradiation dose between 0 and 20 J m-2. About 70% of the UVC-induced DNA sensitivity to nuclease S1 was lost after 30 min of repair. These results suggest that flow cytometry may be useful for assessing the heterogeneity of cell response to UV damage.
在用UVC光照射细胞后，对人成纤维细胞DNA对核酸酶S1活性的敏感性进行了原位研究。通过使用流式细胞术，用特异性荧光染料碘化丙啶（PI）对耐核酸酶的DNA部分进行染色来评估DNA消化情况。结果表明，在0至20 J m-2之间，DNA消化对UVC照射剂量呈非线性依赖关系。修复30分钟后，约70%的UVC诱导的DNA对核酸酶S1的敏感性丧失。这些结果表明，流式细胞术可能有助于评估细胞对紫外线损伤反应的异质性。
