Desfarges L, Durrens P, Juguelin H, Cassagne C, Bonneu M, Aigle M
Laboratoire de Génétique, Talence, France.
Yeast. 1993 Mar;9(3):267-77. doi: 10.1002/yea.320090306.
Selection of mutations, based on the suppression of rvs161 delta defects, was performed. Ten mutants were obtained, ranged amongst four complementation groups, named SUR1, SUR2, SUR3 and SUR4. All sur mutations also suppress a mutation in another gene, RVS167, indicating that all six genes are involved in the same biological pathway. The sur mutant cells have abnormal morphologies in stationary phase, i.e. dumbbell-like in sur1, sur2 or sur3 strains and multi-budded in sur4 strains. Several phenotypic characteristics of the physiological suppressors as well as the rvs161 delta strain itself led us to analyse the phospholipid composition of the mutants. The assays show an overall decrease of the phospholipid amounts and modifications in the relative contents of some phospholipid classes in sur mutant cells.
基于对rvs161δ缺陷的抑制作用进行了突变体筛选。获得了10个突变体，分属于4个互补群，命名为SUR1、SUR2、SUR3和SUR4。所有sur突变也抑制另一个基因RVS167中的突变，表明这六个基因都参与同一生物学途径。sur突变体细胞在稳定期具有异常形态，即sur1、sur2或sur3菌株呈哑铃状，sur4菌株呈多芽状。生理抑制子以及rvs161δ菌株本身的几个表型特征促使我们分析突变体的磷脂组成。分析表明，sur突变体细胞中磷脂总量总体下降，某些磷脂类别的相对含量发生了变化。
