Toupin J, Leyva F, Lamoureux G
Ann Immunol (Paris). 1977 Jan-Mar;128(1-2):29-32.
This transformation was studied at various concentrations of PHA and during different periods of incubation. The coelomocytes were separated into adhering and non-adhering cells. The adhering cells were separated into trypsin-sensitive and trypsin-resistant. Results have clearly shown (P less than or equal to 0.01) that the coelomocytes do transform to PHA. Only the trypsin-resistant adhering cells (5-10%) having a high phagocytic activity, were capable of inducing the transformation of the non-adhering coelomocytes. None of these sub-populations of coelomocytes did transform alone. These results reinforce the existence in earthworms of a T-cell like function and perhaps receptors similar to those observed in higher vertebrates and confirm the cooperation of two cell types to achieve this activity.
在不同浓度的PHA以及不同孵育时间段对这种转化进行了研究。体腔细胞被分离为贴壁细胞和非贴壁细胞。贴壁细胞又被分为对胰蛋白酶敏感的和对胰蛋白酶耐受的。结果清楚地表明（P≤0.01）体腔细胞确实会向PHA转化。只有具有高吞噬活性的对胰蛋白酶耐受的贴壁细胞（5%-10%）能够诱导非贴壁体腔细胞的转化。这些体腔细胞亚群中没有一个能单独发生转化。这些结果进一步证明蚯蚓中存在类似T细胞的功能，或许还存在与高等脊椎动物中观察到的类似的受体，并证实了两种细胞类型为实现这种活性而进行的协作。
