Anuradha P, Thampan R V
School of Life Sciences, University of Hyderabad, India.
Arch Biochem Biophys. 1993 May 15;303(1):81-9. doi: 10.1006/abbi.1993.1258.
Two different methods of study have been employed in the analysis of the hormonal regulation of rat uterine collagenases. The first is concerned with the enzyme activity and the second, its synthesis. The data presented in this report reveal that the rat uterine collagenase is under the regulatory influence of both estradiol and progesterone. This fact is first exemplified by the observation that on Day 2 post-ovriectomy, a wave of collagenase synthesis takes place which influences the enzyme activity in the uterus during the next 3-4 days. This peak in collagenase synthesis disappeared in the uteri of rats subjected to ovariectomy and adrenalectomy simultaneously. It indicated that a hormone of adrenal origin was responsible for the enhanced synthesis and activity of collagenase in the uteri of ovariectomized rats. That the hormone involved was progesterone was shown by the subsequent experimental data. The estradiol-mediated enhancement in rat uterine collagenase activity has been shown to be inhibited by intraluminal exposure of the uteri to actinomycin D/cyclohexinmide, indicating the apparent influence of the hormone at the level of the collagenase gene.
在分析大鼠子宫胶原酶的激素调节过程中采用了两种不同的研究方法。第一种涉及酶活性，第二种涉及酶的合成。本报告中呈现的数据表明，大鼠子宫胶原酶受雌二醇和孕酮的调节影响。这一事实首先通过以下观察得到例证：在卵巢切除术后第2天，会发生一波胶原酶合成，这会在接下来的3 - 4天影响子宫中的酶活性。在同时进行卵巢切除和肾上腺切除的大鼠子宫中，胶原酶合成的这一峰值消失了。这表明肾上腺来源的一种激素负责去卵巢大鼠子宫中胶原酶合成和活性的增强。随后的实验数据表明所涉及的激素是孕酮。已证明，通过向子宫腔内暴露放线菌素D/环己酰亚胺可抑制雌二醇介导的大鼠子宫胶原酶活性增强，这表明该激素在胶原酶基因水平有明显影响。
