Horii A, Konishi E, Urata Y, Azumi Y, Kubo H, Yamagishi H, Oka T, Ashihara T
Dept. of Pathology, Kyoto Prefectural University of Medicine.
Gan To Kagaku Ryoho. 1993 Apr;20(6):808-11.
To study the cell characteristics of relatively small pancreatoduodenal cancers (less than 4 cm in the greatest dimension in histopathological examination), we analyzed the relation between the cell kinetics, and histopathological and immunohistochemical findings. Tumors were classified into t1 (0-2 cm in its greatest dimension) and t2 (2-4 cm). We analyzed DNA contents of five tumors of t1 and twelve of t2 by cytofluorometry. Histopathological and immunohistochemical (CA19-9 and CEA) examinations were also carried out. Nuclear DNA content histograms of the tumors in the t1 group showed all diploid pattern, while those in the t2 group exhibited 4 diploid, 7 euploid polyploid and 1 aneuploid patterns. Invasions to the lymphatic (ly), vein (v) and nerve (pn) were found in five cases of diploid tumors (3 of t1 and 2 of t2) and all (8 in t2) of non-diploid tumors. A medullary pattern of tumor growth was only noted in three tumors of t1. This pattern was not found in t2 (4 diploid, 8 non-diploid). These results suggest that the cellular DNA content increased in association with the tumor growth in size. In the histopathological findings, both the invasions (ly, v, pn) and fibrosis of the stroma more often appeared as the tumor size and polyploid and aneuploid cells increased. However, other histopathological findings and the immunohistochemical examination for CA19-9 and CEA had no relation to the tumor growth and the DNA ploidy pattern.
为研究相对较小的胰十二指肠癌（组织病理学检查中最大径小于4 cm）的细胞特征，我们分析了细胞动力学与组织病理学及免疫组化结果之间的关系。肿瘤分为t1（最大径0 - 2 cm）和t2（最大径2 - 4 cm）。我们通过细胞荧光测定法分析了5例t1肿瘤和12例t2肿瘤的DNA含量。还进行了组织病理学和免疫组化（CA19 - 9和CEA）检查。t1组肿瘤的核DNA含量直方图均显示二倍体模式，而t2组的显示4例二倍体、7例整倍体多倍体和1例非整倍体模式。在5例二倍体肿瘤（t1组3例，t2组2例）和所有（t2组8例）非二倍体肿瘤中发现有淋巴管（ly）、静脉（v）和神经（pn）侵犯。仅在3例t1肿瘤中观察到髓样肿瘤生长模式。t2组（4例二倍体，8例非二倍体）未发现这种模式。这些结果表明，细胞DNA含量随肿瘤大小的增加而增加。在组织病理学结果中，随着肿瘤大小以及多倍体和非整倍体细胞数量的增加，间质的侵犯（ly、v、pn）和纤维化更常出现。然而，其他组织病理学结果以及CA19 - 9和CEA的免疫组化检查与肿瘤生长和DNA倍体模式无关。
