Itoh T, Yamamoto Y, Saka T, Takahashi H
Dept. of Otolaryngology, Osaka Medical College.
Gan To Kagaku Ryoho. 1993 Apr;20(6):839-42.
Eleven tongue papillomas and 25 tongue carcinomas were produced in rats by the oral administration of 0.001% 4-nitroquinoline 1-oxide (4 NQO) in drinking water, and tumor kinetics were investigated by both DNA cytofluorometry and Ag-nucleolar organizer regions (Ag-NORs) staining. The histopathologic characteristics, the mean Ag-NOR number per cell and DNA ploidy patterns were compared. The mean Ag-NOR number was least in normal epithelium, more numerous in papillomas and highest in squamous cell carcinomas. The differences were statistically significant. All cases of normal epithelium and papilloma showed a diploid pattern. Eighteen cases of the squamous cell carcinomas (72%) showed a diploid pattern, and 7 cases (28%) exhibited a diploid plus tetraploid pattern. There was a significant difference in the mean Ag-NOR number between the two groups of squamous cell carcinomas. These results suggest that the mean Ag-NOR number may reflect the cell kinetics in the process of carcinogenesis and polyploidization of carcinomas.
通过在饮水中口服0.001%的4-硝基喹啉-1-氧化物（4NQO），在大鼠中诱发了11个舌乳头状瘤和25个舌癌，并通过DNA细胞荧光测定法和银染核仁组成区（Ag-NORs）染色研究了肿瘤动力学。比较了组织病理学特征、每个细胞的平均Ag-NOR数量和DNA倍体模式。正常上皮中的平均Ag-NOR数量最少，乳头状瘤中较多，鳞状细胞癌中最高。差异具有统计学意义。所有正常上皮和乳头状瘤病例均显示二倍体模式。18例鳞状细胞癌（72%）显示二倍体模式，7例（28%）表现为二倍体加四倍体模式。两组鳞状细胞癌之间的平均Ag-NOR数量存在显著差异。这些结果表明，平均Ag-NOR数量可能反映了癌变过程和癌多倍体化过程中的细胞动力学。
