Gilligan P H, Walden T P, Kelly W F, Wait K J, Kraft J A, Willis D H
Clinical Microbiology/Immunology Laboratories, University of North Carolina Hospital, Chapel Hill 27599.
Arch Pathol Lab Med. 1993 May;117(5):507-10.
A commercially available enzyme immunoassay (EIA) for the detection of Clostridium difficile toxin A (Premier Toxin A EIA, Meridian Diagnostics, Cincinnati, Ohio) was compared with tissue culture cytotoxicity assay, enterotoxigenic culture, and latex agglutination test for the laboratory diagnosis of C difficile-associated disease. When evaluated for detection of C difficile-associated disease using clinical specimens, EIA was the most sensitive (83.1%) and tissue culture cytotoxicity assay was the most specific test with EIA, tissue culture cytotoxicity assay and enterotoxigenic culture having similar correlation values (96.6, 96.1, 94.0%, respectively). The latex agglutination test was not as accurate (89.7% correlation) as the other three tests due mainly to its poor sensitivity (47.9%). The EIA is a rapid, easy-to-use alternative to tissue culture cytotoxicity assay for detection of C difficile-associated disease.
将一种用于检测艰难梭菌毒素A的市售酶免疫测定法（Premier毒素A酶免疫测定法，Meridian诊断公司，俄亥俄州辛辛那提）与组织培养细胞毒性测定法、产肠毒素培养法和乳胶凝集试验进行比较，以用于艰难梭菌相关疾病的实验室诊断。当使用临床标本评估对艰难梭菌相关疾病的检测时，酶免疫测定法最敏感（83.1%），组织培养细胞毒性测定法是最具特异性的检测方法，酶免疫测定法、组织培养细胞毒性测定法和产肠毒素培养法具有相似的相关性值（分别为96.6%、96.1%、94.0%）。乳胶凝集试验不如其他三种检测准确（相关性为89.7%），主要是因为其灵敏度较差（47.9%）。酶免疫测定法是一种快速、易于使用的替代方法，可用于替代组织培养细胞毒性测定法来检测艰难梭菌相关疾病。
