Baier-Bitterlich G, Schumacher W, Schulz T F, Wachter H, Dierich M P
Institut für Hygiene, University of Innsbruck, Austria.
Arzneimittelforschung. 1993 Mar;43(3):395-8.
The effect of penicillamine on the proliferation and viability of human T, B and premacrophage/promyelocytic cell lines was evaluated. Whereas Raji cells and U937 cells were not negatively influenced by the addition of 0.03-0.97 mmol/l D-penicillamine (D-Pen, Trolovol, CAS 52-67-5), other cell lines such as H9, NC37 and HL60 were clearly impaired by concentrations between 0.12 and 0.49 mmol/l. Similar effects on the uptake of 3H-thymidine, 3H-uridine and 3H-leucine were observed with the addition of other stereoisomeric forms of penicillamine, D,L- and L-penicillamine to H9 cell cultures. The evaluation of cell number and viability by light microscopy revealed similar actions of penicillamine on cell cultures. By these means its repressing effect on Jurkat, NC37 and even on U937 became more transparent.
评估了青霉胺对人T、B以及前巨噬细胞/早幼粒细胞系增殖和活力的影响。虽然添加0.03 - 0.97 mmol/l的D - 青霉胺（D - Pen，曲洛钒，CAS 52 - 67 - 5）对拉吉细胞和U937细胞没有负面影响，但其他细胞系，如H9、NC37和HL60，在浓度为0.12至0.49 mmol/l时明显受到损害。在H9细胞培养物中添加青霉胺的其他立体异构体形式，即D，L - 青霉胺和L - 青霉胺时，观察到对3H - 胸腺嘧啶核苷、3H - 尿苷和3H - 亮氨酸摄取的类似影响。通过光学显微镜评估细胞数量和活力，发现青霉胺对细胞培养物有类似作用。通过这些方法，其对Jurkat、NC37甚至U937的抑制作用变得更加明显。
