He D, Karas E, Sassa S, Lim H W
Dermatology Service, Department of Veterans Affairs Medical Center, New York, NY 10010.
Skin Pharmacol. 1993;6(1):20-5. doi: 10.1159/000211080.
Incubation of murine epidermal cells with delta-aminolevulinic acid (ALA) resulted in a dose- and time-dependent accumulation of porphyrins, predominantly of protoporphyrin. Porphyrin accumulation decreased in the presence of iron, and the iron-mediated decrease was partially reversed by CaMg EDTA (1.25-10.0 mM), suggesting that there is functionally active ferrochelatase in these cells. This study suggests that these cells may be a useful model for the study of cutaneous porphyrin metabolism involving ferrochelatase activity.
用δ-氨基乙酰丙酸（ALA）孵育小鼠表皮细胞，导致卟啉呈剂量和时间依赖性积累，主要是原卟啉。在铁存在的情况下，卟啉积累减少，而CaMg EDTA（1.25 - 10.0 mM）可部分逆转铁介导的减少，这表明这些细胞中存在功能活跃的亚铁螯合酶。本研究表明，这些细胞可能是研究涉及亚铁螯合酶活性的皮肤卟啉代谢的有用模型。
