Bauernfeind M, Wood W G
Immunodiagnostic Evaluation Dept., Boehringer Mannheim, Germany.
Eur J Clin Chem Clin Biochem. 1993 Mar;31(3):165-72. doi: 10.1515/cclm.1993.31.3.165.
The following analytes were determined with the automated enzyme immunoassay unit, Enzymun-Test System ES 300, and by routine in-house tests, and the results were compared: thyrotropin, free thyroxine, cortisol, immunoglobulin E, digoxin, digitoxin, insulin and carcinoembryonic antigen. The methods used for comparison included two radioimmunoassays, one with enzyme labelling, two with fluorescence enhanced enzyme reactions and 3 luminescence immunoassays. In most cases, the precision of the ES 300 lay between 2 and 5% in all assays in the concentration ranges of clinical interest. The inter-assay variation was almost identical to the intra-assay precision, which reflects the constancy of reagent quality and machine performance. The correlation between in-house and Enzymun-Test was excellent, even though the slope of the regression line was sometimes far from unity, due to the calibration materials used. ES 300 can be used as a stand alone unit and connected to a host computer. As a "walk-away" machine, it is suitable for laboratories with medium length series. It is a multi-batch analyser capable of taking 12 analytes and a maximal series length of 136 tubes excluding standards and controls.
使用自动酶免疫分析单元Enzymun-Test System ES 300并通过常规内部检测对以下分析物进行了测定，并对结果进行了比较：促甲状腺激素、游离甲状腺素、皮质醇、免疫球蛋白E、地高辛、洋地黄毒苷、胰岛素和癌胚抗原。用于比较的方法包括两种放射免疫分析，一种是酶标记法，两种是荧光增强酶反应法，以及三种发光免疫分析。在大多数情况下，在临床关注的浓度范围内，ES 300在所有检测中的精密度在2%至5%之间。批间变异几乎与批内精密度相同，这反映了试剂质量和仪器性能的稳定性。尽管由于所使用的校准材料，回归线的斜率有时远非1，但内部检测与Enzymun-Test之间的相关性非常好。ES 300可以作为独立单元使用，并连接到主机。作为一台“无需值守”的仪器，它适用于进行中等批量检测的实验室。它是一台多批次分析仪，能够检测12种分析物，最大样本系列长度为136管，不包括标准品和对照品。
