使用重组缺陷型宿主稳定含有植物DNA的酵母人工染色体。
Stabilisation of a yeast artificial chromosome containing plant DNA using a recombination-deficient host.
作者信息
Dunford R, Vilageliu L, Moore G
机构信息
Cambridge Laboratory, John Innes Centre, Norwich, UK.
出版信息
Plant Mol Biol. 1993 Mar;21(6):1187-9. doi: 10.1007/BF00023615.
PMID:8490137
Abstract
The large genomes of many plant species contain numerous dispersed repeat sequences. The problem of large-scale structural instability due to the presence of such repeats in yeast artificial chromosome (YAC) clones was assessed. The feasibility of stabilising plant sequences prone to rearrangement in YACs was demonstrated using a host yeast strain deficient in recombination.
摘要
许多植物物种的庞大基因组包含大量分散的重复序列。评估了由于酵母人工染色体（YAC）克隆中存在此类重复序列而导致大规模结构不稳定的问题。使用缺乏重组的宿主酵母菌株证明了稳定YAC中易于重排的植物序列的可行性。
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