Ryte A, Morelli S, Mazzei M, Alama A, Franco P, Canti G F, Nicolin A
Department of Pharmacology, University of Milan, Italy.
Anticancer Drugs. 1993 Apr;4(2):197-200. doi: 10.1097/00001813-199304000-00011.
A subline of the human B cell lymphoma DHL-4, grown in the artificial serum-free medium HB101, displayed a resistant phenotype to the activity of an antisense oligodeoxynucleotide (aODN) effective on the parental DHL-4 line. It was found that the cellular uptake of the 18mer aODN in the two cell lines was almost the same. In contrast, the unresponsive subline DHL-4r degraded the aODN very efficiently, in contrast to the stability of aODN inside cells of the parental DHL-4 line. Activation of the degrading 'machinery' combined with selective properties of the artificial medium may be responsible for the loss of responsiveness to aODN.
在无血清人工培养基HB101中培养的人B细胞淋巴瘤DHL-4的一个亚系，对一种对亲代DHL-4细胞系有效的反义寡脱氧核苷酸（aODN）的活性表现出抗性表型。结果发现，这两种细胞系中18聚体aODN的细胞摄取情况几乎相同。相比之下，无反应的亚系DHL-4r能非常有效地降解aODN，而亲代DHL-4细胞系细胞内的aODN则具有稳定性。降解“机制”的激活与人工培养基的选择性特性可能是导致对aODN失去反应性的原因。
