Wilhelm F, Melzig M, Franke G
Universitäts-Augenklinik Greifswald.
Ophthalmologe. 1993 Apr;90(2):171-3.
Fluorescein diacetate (FDA) is a suitable substance for vital staining of corneal endothelium. Uptake and metabolism is an active process. This means that a fluorescing cell must be functionally intact. The duration of endothelial fluorescence is limited and should be tested in an experimental study. Cultured endothelial cells (calf aorta endothelial cells) were stained by FDA and the loss of fluorescence time registered. We tested corneal buttons in the same way. The cultured endothelial cells showed no fluorescence after 2 h and the corneal buttons after 40-50 min. The fluorescence of FDA-stained endothelial cells decreases both in cultured cells and in whole corneal buttons, but this is not a sign of loss of endothelial viability. This fact must be taken into consideration when interpreting the results of FDA staining.
荧光素二乙酸酯（FDA）是一种适用于角膜内皮细胞活体染色的物质。摄取和代谢是一个活跃的过程。这意味着发出荧光的细胞必须功能完好。内皮细胞荧光的持续时间是有限的，应该在实验研究中进行测试。用FDA对培养的内皮细胞（小牛主动脉内皮细胞）进行染色，并记录荧光消失的时间。我们以同样的方式测试了角膜植片。培养的内皮细胞在2小时后不再有荧光，角膜植片在40 - 50分钟后不再有荧光。FDA染色的内皮细胞的荧光在培养细胞和整个角膜植片中都会减弱，但这并不是内皮细胞活力丧失的标志。在解释FDA染色结果时必须考虑到这一事实。
