Itoh N, Morinaga N, Kouzai T
Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, Japan.
Biochem Mol Biol Int. 1993 Mar;29(4):785-91.
Nonheme bromoperoxidase found in Pseudomonas putida catalyzed the bromination of aniline with hydrogen peroxide and bromide ions to give o- and p-bromoanilines. However, in the absence of bromide ions, it oxidized aniline via azobenzene and azoxybenzene finally into nitrobenzene. This is the first report of the biological oxidation of an arylamine to the corresponding nitrocompound at the enzyme level. In addition, nitrobenzene was not formed by a nonheme bromoperoxidase from Corallina pilulifera (marine alga), implying that the alga enzyme has a different reaction mechanism.
恶臭假单胞菌中发现的非血红素溴过氧化物酶可催化苯胺与过氧化氢和溴离子发生溴化反应，生成邻溴苯胺和对溴苯胺。然而，在没有溴离子的情况下，它会通过偶氮苯和氧化偶氮苯最终将苯胺氧化为硝基苯。这是关于芳胺在酶水平上生物氧化为相应硝基化合物的首次报道。此外，海膜（一种海藻）中的非血红素溴过氧化物酶不会生成硝基苯，这意味着海藻酶具有不同的反应机制。
