Domer F R, Kaiser L R
Arch Int Pharmacodyn Ther. 1977 Jan;225(1):17-24.
In rabbits anesthetized with urethane, only a small fraction of 3H-methotrexate administered by an i.v. infusion reached an artificial cerebrospinal fluid solution perfused through the cerebroventricular system. This movement was not altered significantly by probenecid administered either i.v. or in the perfusing artificial cerebrospinal fluid. When the 3H-methotrexate was perfused through the cerebroventricular system, one third of the amount normally lost during the perfusion was retained when probenecid was also added to the solution. Therefore, at least a portion of the apparent blood-brain barrier that exists for methotrexate is due to removal from the cerebrospinal fluid by a process that can be inhibited by probenecid.
在用氨基甲酸乙酯麻醉的兔子中，通过静脉输注给予的3H-甲氨蝶呤只有一小部分到达通过脑室系统灌注的人工脑脊液溶液中。静脉注射或在灌注的人工脑脊液中给予丙磺舒，这种转运没有显著改变。当3H-甲氨蝶呤通过脑室系统灌注时，在溶液中加入丙磺舒后，灌注期间通常损失的三分之一的量被保留下来。因此，甲氨蝶呤存在的明显血脑屏障至少有一部分是由于丙磺舒可以抑制的过程从脑脊液中清除所致。
