Passmore J C, Hartupee D A, Jimenez A E, Young J S
Department of Physiology and Biophysics, School of Medicine, University of Louisville, Kentucky 40292.
Circ Shock. 1993 Feb;39(2):107-13.
To investigate the fact that, despite its renal vasodilator properties, acetylcholine (ACh) provides no protection against acute renal failure, we measured nutrient (NBF) and nonnutrient renal blood flow (NNBF) during ACh infusion. The effect of ACh and secretin on NBF in the outer and inner cortex and outer medulla using 133Xenon (133Xe) washout with freeze dissection analysis was determined. We then calculated NNBF as the difference between NBF in the entire cortex and outer medulla (133Xe washout) and total renal blood flow (TRBF) measured by electromagnetic flow probe. NNBF was also assessed from the 86Rubidium (86Rb) recovery after administration into the renal artery. ACh increased TRBF without increasing NBF, resulting in an increased calculated NNBF. ACh, but not secretin, increased NNBF in the kidney as measured with 86Rb. Thus we conclude that ACh selectively induces a large increase in NNBF in the kidney.
为了研究尽管乙酰胆碱（ACh）具有肾血管舒张特性，但它却不能预防急性肾衰竭这一事实，我们在输注ACh期间测量了营养性肾血流量（NBF）和非营养性肾血流量（NNBF）。采用氙-133（133Xe）洗脱冷冻切片分析法，测定了ACh和促胰液素对肾外皮质、肾内皮质和肾外髓质NBF的影响。然后，我们将NNBF计算为整个皮质和肾外髓质的NBF（133Xe洗脱）与通过电磁血流探头测量的总肾血流量（TRBF）之间的差值。NNBF也通过向肾动脉注射铷-86（86Rb）后的回收情况进行评估。ACh增加了TRBF，但未增加NBF，导致计算出的NNBF增加。用86Rb测量时，ACh而非促胰液素增加了肾脏的NNBF。因此，我们得出结论，ACh可选择性地使肾脏的NNBF大幅增加。
