[动物实验中放射性标记细菌使用的问题。1. 用³H、¹⁴C、³²P、⁵⁹Fe、⁹⁹ᵐTc、¹²⁵I标记多杀巴斯德菌、溶血巴斯德菌和都柏林沙门氏菌]

[Problems in the use of radioactively marked bacteria in animal experiments. 1. Labeling of Pasteurella multocida, Pasteurella haemolytica and Salmonella dublin with eH, 14C, 32P, 59Fe, 99mTc, 125J1].

作者信息

Flossmann K D, Rohrmann B, Hubald J, Finsterbusch L

出版信息

Arch Exp Veterinarmed. 1977;31(1):145-59.

Abstract

Several methods are suggested by which to use the radionuclides 3H, 14C, 32P, 59Fe, 99mTc, and 125J for labelling or doublelabelling of Pasteurella multocida, Pasteurella haemolytica, and Salmonella dublin, with particular reference being made to labelling ofr animal experiments. Suitable radioactive substrates for internal labelling in chemically defined or partially defined nutritive media include 3H-thymin, 3H-thymidine, 14C-glucose, 14C-mannose, 14C-aspartic acid, as well as 3H-uracil, 3H-uridine, 3H-orotic acid, 14C-orotic acid, 59Fe-III-citrate or chloride, and Na2H32PO4. The choise of the nuclide and substrate should by governed by the problem at hand.

摘要

文中提出了几种利用放射性核素³H、¹⁴C、³²P、⁵⁹Fe、⁹⁹ᵐTc和¹²⁵I对多杀性巴氏杆菌、溶血巴氏杆菌和都柏林沙门氏菌进行标记或双重标记的方法，特别提及了用于动物实验的标记。在化学成分确定或部分确定的营养培养基中进行内部标记的合适放射性底物包括³H-胸腺嘧啶、³H-胸腺嘧啶核苷、¹⁴C-葡萄糖、¹⁴C-甘露糖、¹⁴C-天冬氨酸，以及³H-尿嘧啶、³H-尿苷、³H-乳清酸、¹⁴C-乳清酸、⁵⁹Fe-III-柠檬酸盐或氯化物，还有Na₂H³²PO₄。核素和底物的选择应根据手头的问题来决定。

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