反相高效液相色谱法、毛细管区带电泳法和胶束毛细管电泳法在分离神经垂体肽及其类似物中的选择性比较。

Comparison of selectivities of reversed-phase high-performance liquid chromatography, capillary zone electrophoresis and micellar capillary electrophoresis in the separation of neurohypophyseal peptides and analogues.

作者信息

Sutcliffe N, Corran P H

机构信息

Chemistry Division, National Institute for Biological Standards and Control, South Mimms, Herts, UK.

出版信息

J Chromatogr. 1993 Apr 23;636(1):95-103. doi: 10.1016/0021-9673(93)80061-c.

DOI:10.1016/0021-9673(93)80061-c

PMID:8491840

Abstract

A number of RP-HPLC systems, including those prescribed in several official monographs, have been evaluated for separating oxytocin, the vasopressins, some clinically important analogues and two additional neurohypophyseal nonapeptides. The separation has been compared with capillary zone electrophoresis and micellar electrophoresis in four micellar systems: cationic, anionic, zwitterionic and neutral. Complete separation was achieved by both RP-HPLC and micellar CE but the importance of charge as a major parameter of separation in CE confers a distinct and useful selectivity to micellar CE based separations.

摘要

已对多种反相高效液相色谱（RP-HPLC）系统进行了评估，包括几部官方专著中规定的系统，用于分离催产素、加压素、一些具有临床重要性的类似物以及另外两种神经垂体九肽。已将该分离方法与毛细管区带电泳和四种胶束系统（阳离子、阴离子、两性离子和中性）中的胶束电泳进行了比较。反相高效液相色谱和胶束毛细管电泳均实现了完全分离，但电荷作为毛细管电泳分离的主要参数，赋予了基于胶束毛细管电泳的分离独特且有用的选择性。