Verstuyf A, Goebels J, Sobis H, Vandeputte M
Rega Institute for Medical Research, University of Leuven, Belgium.
Tumour Biol. 1993;14(1):46-54. doi: 10.1159/000217824.
The influence of epidermal growth factor, insulin-like growth factors I and II, insulin, transforming growth factor beta 1 and transferrin on the growth of a postgestational rat choriocarcinoma was examined by the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide] assay. The cell line was cultured in RPMI 1640 medium supplemented with fetal calf serum, beta-mercaptoethanol, glucose, sodium pyruvate and antibiotics. The experiments were done in media supplemented with 10% (optimal) or 3% (suboptimal) fetal calf serum. Among the different growth factors tested, only epidermal growth factor and to a certain extent insulin had a growth-promoting effect by themselves. The other growth factors had either an additive effect in the presence of epidermal growth factor or no effect at all. The cytotrophoblast cells expressed both epidermal growth factor and transferrin receptors whereas the more differentiated giant cells expressed only transferrin receptors.
采用MTT[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑]分析法，检测了表皮生长因子、胰岛素样生长因子I和II、胰岛素、转化生长因子β1及转铁蛋白对孕后大鼠绒毛膜癌生长的影响。该细胞系在补充有胎牛血清、β-巯基乙醇、葡萄糖、丙酮酸钠及抗生素的RPMI 1640培养基中培养。实验在补充有10%（最佳）或3%（次最佳）胎牛血清的培养基中进行。在测试的不同生长因子中，只有表皮生长因子以及在一定程度上胰岛素自身具有促生长作用。其他生长因子要么在表皮生长因子存在时具有相加作用，要么根本没有作用。细胞滋养层细胞同时表达表皮生长因子和转铁蛋白受体，而分化程度更高的巨细胞仅表达转铁蛋白受体。
