Lin L F, Doherty D H, Lile J D, Bektesh S, Collins F
Synergen, Inc., Boulder, CO 80301.
Science. 1993 May 21;260(5111):1130-2. doi: 10.1126/science.8493557.
A potent neurotrophic factor that enhances survival of midbrain dopaminergic neurons was purified and cloned. Glial cell line-derived neurotrophic factor (GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. In embryonic midbrain cultures, recombinant human GDNF promoted the survival and morphological differentiation of dopaminergic neurons and increased their high-affinity dopamine uptake. These effects were relatively specific; GDNF did not increase total neuron or astrocyte numbers nor did it increase transmitter uptake by gamma-aminobutyric-containing and serotonergic neurons. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.
一种增强中脑多巴胺能神经元存活的强效神经营养因子被纯化并克隆出来。胶质细胞系源性神经营养因子（GDNF）是一种糖基化的、通过二硫键结合的同二聚体，它是转化生长因子-β超家族中关系较远的一个成员。在胚胎中脑培养物中，重组人GDNF促进了多巴胺能神经元的存活和形态分化，并增加了它们对多巴胺的高亲和力摄取。这些作用相对具有特异性；GDNF既没有增加神经元或星形胶质细胞的总数，也没有增加含γ-氨基丁酸和5-羟色胺能神经元的递质摄取。GDNF可能在帕金森病的治疗中具有应用价值，帕金森病的特征是中脑多巴胺能神经元进行性退化。
