Koudela B, Hermánek J
Institute of Parasitology, Academy of Sciences of Czech Republic, Ceské Budĕjovice.
Ann Parasitol Hum Comp. 1993;68(1):3-10. doi: 10.1051/parasite/19936813.
The influence of nonspecific immunomodulation with Thymomodulin (a calf thymic extract with immunomodulatory activity) and hydrocortisone on the course and location of Cryptosporidium parvum infection in neonatal BALB/c mice (infected with 10(6) or 10(5) oocysts on day 7 of life) was studied using scanning electron microscopy of the inner surface of different parts of intestine. Daily peroral treatment of suckling mice with 20 mg/kg/day of Thymomodulin for 5 days before inoculation resulted in an earlier peak and earlier termination of cryptosporidial infection when compared with control infected mice. On the other hand, peroral administration of 25 mg/kg of hydrocortisone every second day led to the persistence of cryptosporidial infection in the ileum of immunosuppressed mice until the end of observation (day 15 post infection), whereas only transient infection was observed in the intestine of control infected mice. The location of infection was also altered in hydrocortisone--treated mice--the severe infection was observed in more proximal parts of the intestine (anterior and middle jejunum), whereas no cryptosporidia were found in these parts of the intestine in nontreated infected mice.
研究了用胸腺调节素（一种具有免疫调节活性的小牛胸腺提取物）和氢化可的松进行非特异性免疫调节对新生BALB/c小鼠（出生第7天感染10⁶或10⁵个卵囊）隐孢子虫感染过程和感染部位的影响，采用对肠道不同部位内表面进行扫描电子显微镜检查的方法。在接种前5天，每天给乳鼠经口投喂20mg/kg/天的胸腺调节素，与对照感染小鼠相比，隐孢子虫感染的峰值出现更早且感染终止更早。另一方面，每隔一天经口给予25mg/kg的氢化可的松导致免疫抑制小鼠回肠中的隐孢子虫感染持续到观察结束（感染后第15天），而对照感染小鼠的肠道中仅观察到短暂感染。在氢化可的松处理的小鼠中感染部位也发生了改变——在肠道更近端的部位（空肠前部和中部）观察到严重感染，而在未处理的感染小鼠的这些肠道部位未发现隐孢子虫。
