Sullia S B, Griffin D H
Biochim Biophys Acta. 1977 Mar 2;475(1):14-22. doi: 10.1016/0005-2787(77)90334-3.
The effects of cycloheximide and related glutarimide antibiotics on DNA synthesis in Achlya bisexualis Coker and A. Couch were compared to those of other protein synthesis inhibitors, puromycin, p-fluorophenylalanine and blasticidin-S. The inhibitors had no significant effects on intrahyphal pool sizes of dTTP, dCTP, ATP, UTP and CTP, nor on the specific activity of the dTTP pool labelled by [3H] thymidine. DNA was the sole acid-insoluble product of [3H]-thymidine incorporation. Cycloheximide, isocycloheximide, streptimidone and blasticidin-S inhibited DNA synthesis rapidly and completely and anhydrocycloheximide was less effective. Cycloheximide acetate, puromycin and p-fluorophenylalanine did not inhibit DNA synthesis. It is concluded that the effects of the several glutarimide antibiotics and of blasticidin-S on DNA synthesis were independent of their effects on protein synthesis.
将环己酰亚胺及相关戊二酰亚胺类抗生素对双性绵霉（Achlya bisexualis Coker）和库氏绵霉（A. Couch）DNA合成的影响，与其他蛋白质合成抑制剂嘌呤霉素、对氟苯丙氨酸和杀稻瘟菌素-S的影响进行了比较。这些抑制剂对菌丝内dTTP、dCTP、ATP、UTP和CTP的库大小，以及由[3H]胸苷标记的dTTP库的比活性均无显著影响。DNA是[3H] - 胸苷掺入的唯一酸不溶性产物。环己酰亚胺、异环己酰亚胺、链霉咪唑酮和杀稻瘟菌素-S迅速且完全地抑制了DNA合成，而脱水环己酰亚胺的效果较差。醋酸环己酰亚胺、嘌呤霉素和对氟苯丙氨酸不抑制DNA合成。得出的结论是，几种戊二酰亚胺类抗生素和杀稻瘟菌素-S对DNA合成的影响与其对蛋白质合成的影响无关。
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