Yoshida S, Cavalieri L F
Biochim Biophys Acta. 1977 Mar 2;475(1):42-53. doi: 10.1016/0005-2787(77)90337-9.
We have shown that a membrane fraction prepared from isolated human lymphoid nuclei contains endogenous DNA-synthesizing activity which is sensitive to RNAase. We have isolated a DNA polymerase from this fraction and partially purified it to what we estimate as about 10 000-fold. Its chromatographic behavior, template specificity, sedimentation constant, pH optimum, and sensitivity to N-ethylmaleimide suggest that the activity resembles but is not identical to DNA polymerase gamma (formerly called R-DNA polymerase). The membrane fraction also contains a minor activity which is due to polymerase beta, the low molecular weight (3.5 S) nuclear enzyme.
我们已经证明，从分离出的人淋巴细胞核制备的膜部分含有对RNA酶敏感的内源性DNA合成活性。我们从该部分分离出一种DNA聚合酶，并将其部分纯化至我们估计约为10000倍的程度。其色谱行为、模板特异性、沉降常数、最适pH值以及对N-乙基马来酰亚胺的敏感性表明，该活性类似于但不同于DNA聚合酶γ（以前称为R-DNA聚合酶）。该膜部分还含有一种次要活性，这是由低分子量（3.5S）核酶——聚合酶β引起的。
