Kotloff D B, Bosma M J, Ruetsch N R
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111.
Int Immunol. 1993 Apr;5(4):383-91. doi: 10.1093/intimm/5.4.383.
Four Pre-B cell clones with intracellular mu chains were recovered from individual leaky scid mice by transformation of bone marrow or peritoneal cells with Abelson murine leukemia virus. Three clones were derived from independent bone marrow cell cultures. These express the defective scid recombinase activity and contain truncated mu chains resulting from abnormal and/or incomplete (D to J only) gene rearrangements. A fourth clone was obtained from a peritoneal cell culture and may represent a revertant. It expresses a recombinase activity indistinguishable from that of wild-type cells; one of its rearranged IgH alleles (VDJ+) encodes a normal size mu chain, the other is non-productively rearranged (VDJ-).
通过用阿贝尔森鼠白血病病毒转化骨髓或腹膜细胞，从个体渗漏型重症联合免疫缺陷（scid）小鼠中获得了四个带有细胞内μ链的前B细胞克隆。三个克隆源自独立的骨髓细胞培养物。这些克隆表达有缺陷的scid重组酶活性，并含有因异常和/或不完全（仅从D到J）基因重排而产生的截短μ链。第四个克隆是从腹膜细胞培养物中获得的，可能代表一个回复体。它表达的重组酶活性与野生型细胞的无法区分；其重排的IgH等位基因之一（VDJ+）编码正常大小的μ链，另一个是非生产性重排（VDJ-）。
