Yang R S, Lu K S, Fu W M, Liu T K, Lin-Shiau S Y
Department of Orthopaedics, College of Medicine, National Taiwan University.
Cell Biol Int. 1993 Jan;17(1):75-82. doi: 10.1006/cbir.1993.1007.
Treatment of cultured rat osteoblasts with staurosporine caused a rapid outgrowth of long slender cellular processes and the formation of stellate cells. The number of stellate cells increased with higher concentrations of and longer incubation with staurosporine. Scanning electron microscopy (SEM) revealed that the smooth surface of control polygonal cells became ruffled with many long slender cellular processes, thus increasing the cell surface area. Transmission electron microscopy (TEM) of the stellate cells showed a rich accumulation of large lipid droplets in the cytoplasm. Some lipid droplets had coalesced under the cytoplasmic membrane. We suggest that staurosporine has an effect on the differentiation of cultured rat osteoblasts.
用星形孢菌素处理培养的大鼠成骨细胞会导致细长的细胞突起迅速生长并形成星状细胞。随着星形孢菌素浓度的升高和孵育时间的延长，星状细胞的数量增加。扫描电子显微镜（SEM）显示，对照多边形细胞的光滑表面因许多细长的细胞突起而变得起伏不平，从而增加了细胞表面积。星状细胞的透射电子显微镜（TEM）显示细胞质中大量脂滴丰富聚集。一些脂滴在细胞质膜下融合。我们认为星形孢菌素对培养的大鼠成骨细胞的分化有影响。
