Netzeband J G, Weathers L B, Strahlendorf H K, Strahlendorf J C
Department of Physiology, Texas Tech University Health Sciences Center, School of Medicine, Lubbock 79430.
Brain Res. 1993 Apr 9;608(1):145-9. doi: 10.1016/0006-8993(93)90786-m.
The modulatory effects of serotonin (5-HT) on excitatory amino acid (EAA)-induced excitations of Purkinje cells (PCs) were examined in urethane-anesthetized adult male rats using microiontophoresis and extracellular recordings. Application of 5-HT had minimal effects on the spontaneous firing rates of PCs but depressed excitations elicited by glutamate (Glu), aspartate (Asp), kainate (KA), and quisqualate (QA), and to a lesser extent those of alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA). Excitations induced by the metabotropic EAA agonist, (+-)-1-aminocyclopentane-trans-1,3-dicarboxylate (t-ACPD), were unaffected by 5-HT. In summary, 5-HT depressed EAA-mediated excitations with the following rank order of effectiveness: Glu = Asp = KA = QA > AMPA >> t-ACPD. These findings suggest that 5-HT shows some selectivity in its modulation of EAA-mediated excitations of PCs and thus may serve an important neuromodulatory role in the cerebellum.
在使用微离子电泳和细胞外记录技术的乌拉坦麻醉成年雄性大鼠中，研究了血清素（5-羟色胺，5-HT）对兴奋性氨基酸（EAA）诱导的浦肯野细胞（PCs）兴奋的调节作用。施加5-HT对PCs的自发放电率影响极小，但可抑制由谷氨酸（Glu）、天冬氨酸（Asp）、 kain酸（KA）和quisqualic酸（QA）引发的兴奋，对α-氨基-3-羟基-5-甲基-4-异恶唑丙酸（AMPA）引发的兴奋的抑制作用较小。代谢型EAA激动剂（±）-1-氨基环戊烷-反式-1,3-二羧酸（t-ACPD）诱导的兴奋不受5-HT影响。总之，5-HT抑制EAA介导的兴奋，其有效性顺序如下：Glu = Asp = KA = QA > AMPA >> t-ACPD。这些发现表明，5-HT在调节EAA介导的PCs兴奋方面具有一定的选择性，因此可能在小脑中发挥重要的神经调节作用。
