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不变链的表达同样控制着MHC II类分子对内源性合成抗原和外源性抗原的提呈。

Invariant chain expression similarly controls presentation of endogenously synthesized and exogenous antigens by MHC class II molecules.

作者信息

Lombard-Platet S, Bertolino P, Gimenez C, Humbert M, Gerlier D, Rabourdin-Combe C

机构信息

ENS-CNRS UMR 49, Lyon, France.

出版信息

Cell Immunol. 1993 Apr 15;148(1):60-70. doi: 10.1006/cimm.1993.1091.

DOI:10.1006/cimm.1993.1091
PMID:8495491
Abstract

I-Ak- and I-Ed-transfected L fibroblasts were supertransfected with cDNA coding for hen egg lysozyme (HEL) or measles virus hemagglutinin (HA). Well-defined cell culture conditions allowed us to obtain L cells with either no detectable endogenous Ii mRNA or a high level of endogenous Ii mRNA induced by serum starvation. Cells supertransfected with mouse Ii chain gene stably expressing a high level of Ii chain were also used as APC in parallel experiments. Class II presentation of endogenously secreted HEL or an ER-retained form of HEL to the HEL-specific I-Ak-restricted 3A9 T cell hybridoma was found to be strongly enhanced in cell transfectants expressing Ii chain. Similar results were obtained with the presentation of transmembrane HA to the HA-specific I-Ed-restricted TH5.143 T cell hybridoma. These findings correlate with those obtained with the presentation of exogenous HEL and HA. In addition, as reported to be the case for exogenous antigen, expression of a large amount of endogenous HA by the APC supplants the requirement for Ii chain expression in the enhancement of antigen presentation. These data demonstrate that presentation by MHC class II molecules of a given antigen, either exogenously provided or endogenously synthesized, is controlled in a similar manner by the Ii chain.

摘要

用编码鸡卵溶菌酶（HEL）或麻疹病毒血凝素（HA）的cDNA对转染了I-Ak和I-Ed的L成纤维细胞进行超转染。明确的细胞培养条件使我们能够获得内源性Ii mRNA无法检测到的L细胞，或通过血清饥饿诱导产生高水平内源性Ii mRNA的L细胞。在平行实验中，用稳定表达高水平Ii链的小鼠Ii链基因超转染的细胞也用作抗原呈递细胞（APC）。结果发现，在表达Ii链的细胞转染子中，内源性分泌的HEL或内质网保留形式的HEL向HEL特异性I-Ak限制性3A9 T细胞杂交瘤的II类呈递得到了显著增强。对于跨膜HA向HA特异性I-Ed限制性TH5.143 T细胞杂交瘤的呈递，也得到了类似的结果。这些发现与外源性HEL和HA呈递的结果相关。此外，正如外源性抗原的情况报道那样，APC大量表达内源性HA可替代Ii链表达在增强抗原呈递中的需求。这些数据表明，由MHC II类分子呈递的给定抗原，无论是外源性提供还是内源性合成，都以类似的方式受Ii链控制。

相似文献

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Invariant chain expression similarly controls presentation of endogenously synthesized and exogenous antigens by MHC class II molecules.不变链的表达同样控制着MHC II类分子对内源性合成抗原和外源性抗原的提呈。
Cell Immunol. 1993 Apr 15;148(1):60-70. doi: 10.1006/cimm.1993.1091.
2
Inhibition by chloroquine of the class II major histocompatibility complex-restricted presentation of endogenous antigens varies according to the cellular origin of the antigen-presenting cells, the nature of the T-cell epitope, and the responding T cell.氯喹对内源性抗原的II类主要组织相容性复合体限制提呈的抑制作用，因抗原呈递细胞的细胞来源、T细胞表位的性质以及应答性T细胞的不同而有所差异。
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Correlation between invariant chain expression level and capability to present antigen to MHC class II-restricted T cells.恒定链表达水平与向MHC II类限制性T细胞呈递抗原能力之间的相关性。
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引用本文的文献

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Beyond the classical: influenza virus and the elucidation of alternative MHC class II-restricted antigen processing pathways.超越经典：流感病毒与揭示另类 MHC II 类限制的抗原加工途径。
Immunol Res. 2011 Dec;51(2-3):237-48. doi: 10.1007/s12026-011-8257-3.
2
Inhibition by chloroquine of the class II major histocompatibility complex-restricted presentation of endogenous antigens varies according to the cellular origin of the antigen-presenting cells, the nature of the T-cell epitope, and the responding T cell.氯喹对内源性抗原的II类主要组织相容性复合体限制提呈的抑制作用，因抗原呈递细胞的细胞来源、T细胞表位的性质以及应答性T细胞的不同而有所差异。
Immunology. 1993 Dec;80(4):566-73.