Decoville M, Schwartz A, Locker D, Leng M
Centre de Biophysique Moléculaire, CNRS, Orléans, France.
FEBS Lett. 1993 May 24;323(1-2):55-8. doi: 10.1016/0014-5793(93)81447-8.
Two DNA restriction fragments containing either a d(GC)5 or a d(TTGCTTGATTAGTTGTGTT) insert were subjected to reaction with cis-diamminedichloroplatinum(II) and were then used as templates for RNA synthesis by T7 RNA polymerase. Within the d(GC)5 insert, interstrand cross-links are preferentially formed. Within the second insert, the reactivity order of the potential binding sites is d(ApG) > d(GpC/GpC) = d(GpA) > d(GpTpG). In the presence of cyanide ions, the adducts are much less stable at the d(GpA) sites than at the d(GpCpG) sites, in double-stranded DNA.
两个分别含有d(GC)5或d(TTGCTTGATTAGTTGTGTT)插入片段的DNA限制性片段与顺二氯二氨合铂(II)进行反应，然后用作T7 RNA聚合酶进行RNA合成的模板。在d(GC)5插入片段中，链间交联优先形成。在第二个插入片段中，潜在结合位点的反应活性顺序为d(ApG) > d(GpC/GpC) = d(GpA) > d(GpTpG)。在氰离子存在的情况下，双链DNA中d(GpA)位点的加合物比d(GpCpG)位点的加合物稳定性低得多。
