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[在31摄氏度器官培养基中保存的人角膜超微结构研究]

[Ultrastructural study of human cornea preserved in organ culture media at +31 degrees C].

作者信息

Borderie V, Kantelip B, Delbosc B, Carbillet J P, Piquot X, Montard M, Laroche L

机构信息

Service d'ophtalmologie, CHU Jean-Minjoz, Besançon.

出版信息

J Fr Ophtalmol. 1993;16(2):80-6.

PMID:8496560
Abstract

Organ culture becomes a standard method of corneal graft preservation. The aim of the study was to evaluate the preservation injuries induced by organ culture. We examined 12 organ cultured human corneas. Corneas were preserved in the medium for 2 to 21 days. Corneas showed some abnormalities: numerous light vacuoles, mitochondrial swelling and increased cell thickness in all cells of the cornea, sloughing of the external and medium epithelial cell layers. We observed normal endoplasmic reticulum, Golgi apparatus and nucleus. These preservation injuries are moderate and reversible.

摘要

器官培养成为角膜移植保存的标准方法。本研究的目的是评估器官培养引起的保存损伤。我们检查了12个经器官培养的人角膜。角膜在培养基中保存2至21天。角膜出现了一些异常:大量清亮空泡、线粒体肿胀以及角膜所有细胞的细胞厚度增加、外层和中层上皮细胞层脱落。我们观察到内质网、高尔基体和细胞核正常。这些保存损伤是中度且可逆的。

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