The trypsin and chymotrypsin inhibitors in chick pears (Cicer arietinum L). The relationships among the six isoinhibitors.

The six isoinhibitors of trypsin and chymotrypsin which were previously purified from a crude extract of chick peas were further studied with the intention of establishing the relationships among them. Exposure of each fraction to active, matrix-bound trypsin resulted in a decrease of the specific inhibitory activities towards trypsin of fractions P-1, P-3 and P-5 while those of P-4 and P-6 were increased. Each of these fractions was separated into several sub-fractions after chromatography on a DEAE-Sephadex A-25 column under conditions identical to those used previously for their purification. The elution positions, specific inhibitory activities towards trypsin and electrophoretic patterns in polyacrylamide gels of the various sub-fractions were determined...