Singh G, Chaudry K I, Chaudry I H
Department of Surgery, Michigan State University, East Lansing 48824.
Am J Physiol. 1993 May;264(5 Pt 2):R977-83. doi: 10.1152/ajpregu.1993.264.5.R977.
The aim of this study was to determine whether ATP-MgCl2 as an adjunct to resuscitation has any beneficial effect on the depressed gut absorptive capacity (GAC) after hemorrhage and, if so, whether alterations in whole blood viscosity (WBV) are, in part, responsible for those effects. Rats were anesthetized, a laparotomy was performed (i.e., trauma induced), and blood vessels were cannulated. The animals then underwent fixed pressure (40 mmHg) hemorrhage, followed by resuscitation with lactated Ringer solution. One group received 50 mumol/kg ATP-MgCl2 and another received saline during resuscitation. At 2 and 4 h after the end of hemorrhage, a 1-h D-xylose absorption test was performed. Viscosity determinations were made at corresponding time points in another set of rats subjected to sham hemorrhage, acute hemodilution, or hemorrhage and resuscitation. Results show that xylose absorption is depressed after hemorrhage and resuscitation and that ATP-MgCl2 restored it to normal. D-Xylose absorption was inhibited by glucose, indicating a facilitated transport process. Furthermore, WBV was reduced by the administration of ATP-MgCl2. Thus ATP-MgCl2 improves GAC early after hemorrhage and resuscitation, possibly by reducing WBV, and may be a useful adjunct to resuscitation, allowing for early enteral nutrition.
本研究的目的是确定作为复苏辅助手段的ATP - MgCl₂对出血后降低的肠道吸收能力（GAC）是否有任何有益作用，以及如果有作用，全血粘度（WBV）的改变是否部分地导致了这些作用。将大鼠麻醉，进行剖腹手术（即诱导创伤），并插入血管插管。然后动物接受固定压力（40 mmHg）出血，随后用乳酸林格溶液进行复苏。一组在复苏期间接受50 μmol/kg ATP - MgCl₂，另一组接受生理盐水。在出血结束后2小时和4小时，进行1小时的D - 木糖吸收试验。在另一组接受假出血、急性血液稀释或出血及复苏的大鼠的相应时间点测定粘度。结果表明，出血和复苏后木糖吸收降低，而ATP - MgCl₂将其恢复至正常。葡萄糖抑制D - 木糖吸收，表明存在易化转运过程。此外，给予ATP - MgCl₂可降低WBV。因此，ATP - MgCl₂可能通过降低WBV在出血和复苏后早期改善GAC，并且可能是复苏的有用辅助手段，从而允许早期肠内营养。
