Lenstra J A, van Boxtel J A, Zwaagstra K A, Schwerin M
Institute of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht, The Netherlands.
Anim Genet. 1993 Feb;24(1):33-9. doi: 10.1111/j.1365-2052.1993.tb00916.x.
DNA sequences from Bovidae (cattle, goats and sheep) in the EMBL nucleotide database contain several short interspersed repeated sequences (SINEs). Three different SINEs have been found: Bov-A2, containing two 115-bp A elements; Bov-tA, a tRNA pseudogene coupled to an A element; and Bov-B of 560 bp or less and partially homologous to the A element. Bov-A2, Bov-tA and Bov-B occupy about 1.8%, 1.6% and 0.5%, respectively, of the bovine genome as represented in the nucleotide database. Apart from a tRNA-like sequence in both Bov-tA and the porcine SINEs, there was no similarity with the porcine SINEs. Apparently, the artiodactyle SINEs were established after the divergence leading to the Suidae and Bovidae but before the radiation within these families. Oligonucleotides were designed for a specific amplification of DNA from Bovidae.
欧洲分子生物学实验室核苷酸数据库中牛科动物(牛、山羊和绵羊)的DNA序列包含几种短散在重复序列(SINE)。已发现三种不同的SINE:Bov-A2,包含两个115bp的A元件;Bov-tA,一个与A元件相连的tRNA假基因;以及长度为560bp或更短且与A元件部分同源的Bov-B。在核苷酸数据库所代表的牛基因组中,Bov-A2、Bov-tA和Bov-B分别占约1.8%、1.6%和0.5%。除了Bov-tA和猪SINE中都有的一个类似tRNA的序列外,与猪SINE没有相似性。显然,偶蹄目动物的SINE是在导致猪科和牛科分化之后但在这些科内辐射之前形成的。设计了寡核苷酸用于特异性扩增牛科动物的DNA。