Toshimori K, Oura C
Department of Anatomy, Miyazaki Medical College, Japan.
Arch Histol Cytol. 1993 Mar;56(1):109-16. doi: 10.1679/aohc.56.109.
This report documents ultrastructural changes in the postacrosomal region of the hamster and mouse sperm head at the initial stage of gamete interaction in vivo. Prominent structures were sequentially visualized: first, the periodic substructure crossbridging the postacrosomal sheath to the overlying plasma membrane, and then the small, electron-dense, granular structures lining the outer surface of the postacrosomal sheath. The periodic substructure became visible at the restricted region where the sperm plasma membrane was just prior to or in the act of detaching from the periodic substructure. The granular substances lined up along the external face of the postacrosomal sheath immediately after the detachment of the sperm plasma membrane, but before the complete degradation of the periodic substructure. These structural changes were completed before sperm nuclear decondensation started. The region where the granular structures were visualized was close to that of the antigen recognized by the monoclonal antibody MN13, which is supposed to be involved in the association of the periodic substructure with the overlaying plasma membrane (Toshimor et al., 1991). Based upon these results, we present the progress of events at the initial stage of gamete interaction.
本报告记录了在体内配子相互作用初始阶段仓鼠和小鼠精子头部顶体后区域的超微结构变化。依次观察到了显著的结构：首先是将顶体后鞘与覆盖其上的质膜交联的周期性亚结构，然后是排列在顶体后鞘外表面的小的、电子致密的颗粒状结构。周期性亚结构在精子质膜即将从周期性亚结构脱离或正在脱离的受限区域变得可见。颗粒物质在精子质膜脱离后、周期性亚结构完全降解之前，立即沿着顶体后鞘的外表面排列。这些结构变化在精子核去浓缩开始之前就已完成。可视化颗粒结构的区域与单克隆抗体MN13识别的抗原区域相近，该抗原被认为参与周期性亚结构与覆盖质膜的关联（Toshimor等人，1991年）。基于这些结果，我们展示了配子相互作用初始阶段事件的进展。
