Díez-Guerra F J, Avila J
Centro de Biología Molecular, Facultad de Ciencias, Universidad Autónoma de Madrid, Spain.
Neuroreport. 1993 Apr;4(4):419-22. doi: 10.1097/00001756-199304000-00020.
Cultures of hippocampal neurones have been used to study a possible correlation between the pattern of dendritic growth and post-translational modification of Microtubule-Associated Protein 2 (MAP2). During the first three days in vitro, a small increase in the total amount of MAP2 is observed, whereas the level of phosphorylation increases exponentially, being particularly dramatic after three days in vitro. Analysis of dendrite morphology by MAP2 immunofluorescence, revealed a parallel exponential growth in dendrite arborization with maximum rates at the third day of culture. We propose a correlation between dendrite arborization and phosphorylation of MAP2, that could be mediated by the establishment of cell contacts.
海马神经元培养物已被用于研究树突生长模式与微管相关蛋白2（MAP2）翻译后修饰之间的可能相关性。在体外培养的前三天，观察到MAP2总量有小幅增加，而磷酸化水平呈指数增长，在体外培养三天后尤为显著。通过MAP2免疫荧光分析树突形态，发现树突分支呈平行指数生长，在培养第三天达到最大生长速率。我们提出树突分支与MAP2磷酸化之间存在相关性，这可能由细胞接触的建立介导。
